ERK1 Antikörper (AA 251-300)

Produktnummer ABIN6741660

Der Kaninchen Polyklonal anti-ERK1 Antikörper wird verwendet zum Nachweis von ERK1 in Proben von Human, Maus, Ratte, Schwein, Hund, Rind (Kuh), Affe, Zebrafisch (Danio rerio), Pferd und Meerschweinchen. Er wurde validiert für WB.

Kurzübersicht für ERK1 Antikörper (AA 251-300) (ABIN6741660)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reaktivität: Human, Maus, Ratte, Schwein, Hund, Rind (Kuh), Affe, Zebrafisch (Danio rerio), Pferd, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-ERK1 Antikörper

Bindungsspezifität: AA 251-300

Spezifität: Human MAPK3 / ERK1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse (100%) Rabbit (92%) Zebrafish (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa251-300 of human MAPK3 (P27361, NP_002737). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Marmoset, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig (100%), Elephant, Bat, Rabbit, Opossum (92%), Stickleback (91%), Platypus, Salmon, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Andere Bezeichnung: MAPK3 / ERK1

Hintergrund: Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3

Gen-ID: 5595

NCBI Accession: NP_002737

UniProt: P27361

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteine