AIF Antikörper (AA 211-260)

Produktnummer ABIN6736699

Der Kaninchen Polyklonal anti-AIF Antikörper (ABIN6736699) detektiert spezifisch AIF in WB, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Schwein, Rind (Kuh), Hund, Pferd, Zebrafisch (Danio rerio) und Hamster.

Kurzübersicht für AIF Antikörper (AA 211-260) (ABIN6736699)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reaktivität: Human, Ratte, Maus, Schwein, Rind (Kuh), Hund, Pferd, Zebrafisch (Danio rerio), Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AIF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-AIF Antikörper

Bindungsspezifität: AA 211-260

Spezifität: Human AIFM1 / AIF

Homologie: Percent identity by BLAST analysis:

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa211-260 of human AIFM1 (O95831-2, NP_665812). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Cat, Bovine, Horse, Pig (100%), Monkey, Rabbit, Guinea pig (92%), Opossum, Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Andere Bezeichnung: AIFM1 / AIF / PDCD8

Hintergrund: Name/Gene ID: AIFM1
Family: Apoptosis

Synonyms: AIFM1, AIF, COXPD6, PDCD8, Programmed cell death 8

Gen-ID: 9131

NCBI Accession: NP_665812

UniProt: O95831

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effekt