TCF7 Antikörper (AA 228-277)

Produktnummer ABIN6736432

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch TCF7 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Affe und Fledermaus.

Kurzübersicht für TCF7 Antikörper (AA 228-277) (ABIN6736432)

Target: TCF7 (Transcription Factor 7 (T-Cell Specific, HMG-Box) (TCF7))

Reaktivität: Human, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Affe, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TCF7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-TCF7 Antikörper

Bindungsspezifität: AA 228-277

Marke: IHC-plus™

Spezifität: Human TCF7

Homologie: Percent identity by BLAST analysis: Human, Horse, Guinea pig (100%) Mouse, Rat, Dog, Pig (92%) Bovine, Rabbit (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa228-277 of human TCF7 (Q86WR9, NP_003193). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Bat, Horse, Guinea pig (100%), Galago, Mouse, Rat, Panda, Dog, Pig (92%), Elephant, Bovine, Rabbit (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.12 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/ul.Antibody has been tested in peptide-based ELISA.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu TCF7

Target: TCF7 (Transcription Factor 7 (T-Cell Specific, HMG-Box) (TCF7))

Andere Bezeichnung: TCF-1 / TCF7

Hintergrund: Name/Gene ID: TCF7
Family: Transcription factor

Synonyms: TCF7, TCF-7, TCF1, Transcription factor 7, T-cell factor 1, TCF-1

Gen-ID: 6932

NCBI Accession: NP_003193

UniProt: P36402

Pathways: WNT Signalweg