PDK4 Antikörper (N-Term)

Produktnummer ABIN6736421

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PDK4 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Pferd, Schwein, Kaninchen, Fledermaus und Affe.

Kurzübersicht für PDK4 Antikörper (N-Term) (ABIN6736421)

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Reaktivität: Human, Pferd, Schwein, Kaninchen, Fledermaus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PDK4 Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human PDK4

Homologie: Percent identity by BLAST analysis: Human, Rabbit, Horse, Pig (100%) Mouse, Rat, Bovine, Guinea pig (92%) Dog (91%) Chicken (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human PDK4 (Q16654, NP_002603). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Bat, Rabbit, Horse, Pig, Platypus (100%), Mouse, Rat, Bovine, Guinea pig (92%), Dog (91%), Lizard (90%), Turkey, Chicken (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu PDK4

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Andere Bezeichnung: PDK4

Hintergrund: Name/Gene ID: PDK4
Subfamily: PDK/BCKDK
Family: Protein Kinase

Synonyms: PDK4, PDHK4

Gen-ID: 5166

NCBI Accession: NP_002603

UniProt: Q16654

Pathways: PI3K-Akt Signalweg, Hedgehog Signalweg, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt