PCNA Antikörper (AA 185-261)
Kurzübersicht für PCNA Antikörper (AA 185-261) (ABIN672756)
Target
Alle PCNA Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 185-261
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Kreuzreaktivität
- Huhn, Human, Maus, Kaninchen, Ratte, Schaf
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Homologie
- Dog,Cow
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Aufreinigung
- Purified by Protein A.
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Immunogen
- KLH conjugated synthetic peptide derived from human PCNA
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Isotyp
- IgG
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Applikationshinweise
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 μg/μL
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Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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Konservierungsmittel
- ProClin
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Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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Haltbarkeit
- 12 months
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The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B 1." in: Scientific reports, Vol. 8, Issue 1, pp. 1770, (2018) (PubMed).
: "Long non-coding RNA SPRY4-IT1 promotes the proliferation and invasion of U251 cells through upregulation of SKA2." in: Oncology letters, Vol. 15, Issue 3, pp. 3977-3984, (2018) (PubMed).
: "The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum." in: Oncotarget, Vol. 7, Issue 24, pp. 35592-35606, (2016) (PubMed).
: "Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats." in: Experimental and therapeutic medicine, Vol. 10, Issue 3, pp. 851-856, (2015) (PubMed).
: "Urotensin II promotes atherosclerosis in cholesterol-fed rabbits." in: PLoS ONE, Vol. 9, Issue 4, pp. e95089, (2015) (PubMed).
: "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." in: Theriogenology, (2014) (PubMed).
: "Potential contribution of SOCC to cerebral vasospasm after experimental subarachnoid hemorrhage in rats." in: Brain research, Vol. 1517, pp. 93-103, (2013) (PubMed).
: "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." in: BMC cancer, Vol. 12, pp. 464, (2012) (PubMed).
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The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B 1." in: Scientific reports, Vol. 8, Issue 1, pp. 1770, (2018) (PubMed).
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- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
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Andere Bezeichnung
- PCNA
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Hintergrund
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Synonyms: ATLD2, Proliferating cell nuclear antigen, PCNA, Cyclin
Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
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Gen-ID
- 5111
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UniProt
- P12004
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Pathways
- Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie
Target
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