MECP2 Antikörper (AA 119-453)

Produktnummer ABIN6719426

Der Kaninchen Polyklonal Anti-MECP2-Antikörper wurde für WB, ELISA, IHC, IF und ICC validiert. Er ist geeignet, MECP2 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für MECP2 Antikörper (AA 119-453) (ABIN6719426)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MECP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-MECP2 Antikörper

Bindungsspezifität: AA 119-453

Verwendungszweck: Anti-MECP2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-MECP2 Antibody Picoband® (ABIN6719426). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MECP2 recombinant protein (Position: K119-R453).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Amir RE, Van den Veyver IB, Wan M, Tran CQ, Francke U, Zoghbi HY(October 1999). "Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2". Nat. Genet. 23 (2): 185-8. 2. Chahrour M, et al. (2008). "MECP2, a key contributor to neurological disease, activates and represses transcription". Science. 320 (5880): 1224-9.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Andere Bezeichnung: MECP2

Hintergrund:

Synonyms: Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2

Tissue Specificity: Present in all adult somatic tissues tested.

Background: MECP2 (methyl CpG binding protein 2) is a gene that encodes the protein MECP2. It is mapped to Xq28. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molekulargewicht: 75 kDa

Gen-ID: 4204

UniProt: P51608

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane