CFP Antikörper

Produktnummer ABIN6719298

Dieses Anti-CFP-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CFP in WB, IHC, FACS und IHC (fro). Geeignet für Human, Maus und Ratte.

Kurzübersicht für CFP Antikörper (ABIN6719298)

Target: CFP (Complement Factor P (CFP))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-CFP Antikörper

Verwendungszweck: Anti-CFP Antibody Picoband®

Sequenz: MVEGQGEKNV TFWGRPLPRC EELQGQKLVV EEKR

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-CFP Antibody Picoband® (ABIN6719298). Tested in Flow Cytometry, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CFP, which shares 86.1% amino acid (aa) sequence identity with both mouse and rat CFP.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. Ash, S., Johnson, C., Shohat, M., Shohat, T., Schlesinger, M. Further mapping of the properdin deficiency gene in a Tunisian Jewish family--evidence for genetic homogeneity. Isr. J. Med. Sci. 30: 626-628, 1994. 2. Coleman, M. P., Murray, J. C., Willard, H. F., Nolan, K. F., Reid, K. B. M., Blake, D. J., Lindsay, S., Bhattacharya, S. S., Wright, A., Davies, K. E. Genetic and physical mapping around the properdin P gene. Genomics 11: 991-996, 1991.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CFP

Target: CFP (Complement Factor P (CFP))

Andere Bezeichnung: CFP

Hintergrund:

Synonyms: Properdin, Complement factor P, CFP, PFC

Tissue Specificity: Widely expressed.

Background: Properdin (factor P) is a plasma protein that is active in the alternative complement pathway of the innate immune system. It is mapped to Xp11.23. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Molekulargewicht: 51 kDa

Gen-ID: 5199

UniProt: P27918

Pathways: Komplementsystem