PDK4 Antikörper (AA 311-411) (HRP)

Produktnummer ABIN671865

Dieses Anti-PDK4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PDK4 in WB, ELISA, IHC (p) und IHC (fro). Geeignet für Human und Maus.

Kurzübersicht für PDK4 Antikörper (AA 311-411) (HRP) (ABIN671865)

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDK4 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-PDK4 Antikörper (HRP)

Bindungsspezifität: AA 311-411

Kreuzreaktivität: Human, Maus

Homologie: Rat

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Pdk4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu PDK4

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Andere Bezeichnung: PDK4

Hintergrund:

Synonyms: FLJ40832, Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial, Pyruvate dehydrogenase kinase 4, Pyruvate dehydrogenase kinase isoform 4, Pyruvate dehydrogenase kinase isozyme 4 mitochondrial.

Background: Pdk4 (pyruvate dehydrogenase kinase, isozyme 4) mediates glucose oxidation through inhibitory phosphorylation of the pyruvate dehydrogenase complex. The pyruvate dehydrogenase complex (PDC) is a mitochondrial matrix enzyme that plays an key role in aerobic energy metabolism by catalyzing the oxidative decarboxylation of pyruvate to form acetyl-CoA for subsequent breakdown in the Krebs cycle. The enzymatic activity of the mammalian PDC is regulated through phosphorylation and desphosphorylation of the E1?subunit of the complex. The enzymes responsible for the phosphorylation and inactivation of the PDC are pyruvate dehydrogenase kinases (PDK1-4). [TISSUE SPECI FICITY] Ubiquitous, highest levels of expression in heart and skeletal muscle. Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain.

Gen-ID: 5166

Pathways: PI3K-Akt Signalweg, Hedgehog Signalweg, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt