Cyclin D3 Antikörper (AA 121-220) (HRP)

Produktnummer ABIN671580

Dieses Anti-Cyclin D3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Cyclin D3 in WB, ELISA, IHC (p) und IHC (fro). Geeignet für Human und Kaninchen.

Kurzübersicht für Cyclin D3 Antikörper (AA 121-220) (HRP) (ABIN671580)

Target: Cyclin D3 (CCND3)

Reaktivität: Human, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin D3 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Cyclin D3 Antikörper (HRP)

Bindungsspezifität: AA 121-220

Kreuzreaktivität: Human, Kaninchen

Homologie: Mouse,Rat,Dog,Cow,Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Cyclin D3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Cyclin D3

Target: Cyclin D3 (CCND3)

Andere Bezeichnung: Cyclin D3

Hintergrund:

Synonyms: G1/S-specific cyclin-D3, CCND3

Background: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Gen-ID: 896

UniProt: P30281

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process