CDK1 Antikörper (AA 201-297)

Produktnummer ABIN669891

Dieses Anti-CDK1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CDK1 in WB, ELISA, IHC (p), FACS, IF (cc), IF (p) und IHC (fro). Geeignet für Human, Maus, Ratte, Hund und Schaf. Dieses Primary Antibody wurde in 7+ Publikationen zitiert.

Kurzübersicht für CDK1 Antikörper (AA 201-297) (ABIN669891)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reaktivität: Human, Maus, Ratte, Hund, Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-CDK1 Antikörper

Bindungsspezifität: AA 201-297

Kreuzreaktivität: Hund, Human, Maus, Ratte, Schaf

Homologie: Cow

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse CDK1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-CDK1 Antikörper (ABIN669891)

Ghate, Das, Chaudhuri, Panja, Mandal: "Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio." in: Cell death discovery, Vol. 2, pp. 15062, (2016) (PubMed).

He, Zhang, Gan, Xu, Tang: "Expression of hyperpolarization-activated cyclic nucleotide-gated channel isoforms in a canine model of atrial fibrillation." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 433-436, (2016) (PubMed).

Dai, Ye, Peng, Zhou, Bai, Xiao, Ma, Zhou, Tang, Yao, Cao, Qin, Liu, Tan: "Aptamer TY04 inhibits the growth of multiple myeloma cells via cell cycle arrest." in: Tumour biology, Vol. 35, Issue 8, pp. 7561-8, (2014) (PubMed).

Haolong, Du, Hongchao, Yang, Wei, Hua, Wenliang, Lei, Po: "Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells." in: PLoS ONE, Vol. 8, Issue 9, pp. e73900, (2013) (PubMed).

Ghate, Chaudhuri, Sarkar, Sajem, Panja, Rout, Mandal: "An antioxidant extract of tropical lichen, Parmotrema reticulatum, induces cell cycle arrest and apoptosis in breast carcinoma cell line MCF-7." in: PLoS ONE, Vol. 8, Issue 12, pp. e82293, (2013) (PubMed).

Yang, Gu, Zheng, Li, Lian, Miao, Jiang, Wei: "RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells." in: The Journal of biological chemistry, Vol. 286, Issue 13, pp. 11865-74, (2011) (PubMed).

Details zu CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Andere Bezeichnung: CDK1

Hintergrund:

Synonyms: Cdc2, Cdc2a, p34, Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, Cdkn1

Background: The cell division control protein cdc2, also known as cyclin dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Gen-ID: 12534

UniProt: P11440

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA