Connexin 43/GJA1 Antikörper (pSer368)

Produktnummer ABIN6658233

Hersteller Produkt- Nr.: 612-401-d23

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Connexin 43/GJA1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für Connexin 43/GJA1 Antikörper (pSer368) (ABIN6658233)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Connexin 43/GJA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Connexin 43/GJA1 Antikörper

Bindungsspezifität: pSer368

Hersteller Produkt- Nr.: 612-401-d23

Hersteller: Rockland

Verwendungszweck: Connexin 43 phospho S368 Antibody

Kreuzreaktivität (Details): Anti-Connexin 43 pS368 antibody is directed against rat pS368 Connexin 43

Aufreinigung: .Anti-Connexin 43 pS368 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-Connexin 43 pS368 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Connexin 43 pS368 Antibody has been tested in western blot and is suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 43 kDa in size corresponding to connexin 43 protein phosphorylated at Ser368 in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Andere Bezeichnung: Connexin 43

Hintergrund:

Synonyms: Gap junction alpha-1 protein, Gap junction 43 kDa heart protein, CX43

Background: Anti-Connexin 43 pS368 Antibody detects pS368 Connexin which is thought to play a key role in development and may also be involved in epilepsy. Connexin 43 antibody recognizes Connexin43 which forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C. Phosphorylation of Ser368 by PKC induces a conformational change of connexin 43 that results in a decrease in gap junction permeability. Anti-Connexin 43 pS368 Antibody is ideal for investigators involved in Neuroscience and Cell Signaling.

Gene Name: GJA1

Gen-ID: 24392

UniProt: P08050

Pathways: MAPK Signalweg, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization