MECP2 Antikörper (pSer421)

Produktnummer ABIN6658009

Hersteller Produkt- Nr.: 600-401-x14

Der Kaninchen Polyklonal Anti-MECP2-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, MECP2 in Proben von Maus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für MECP2 Antikörper (pSer421) (ABIN6658009)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MECP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-MECP2 Antikörper

Bindungsspezifität: pSer421

Hersteller Produkt- Nr.: 600-401-x14

Hersteller: Rockland

Verwendungszweck: MeCP2 Phospho S421 Antibody

Kreuzreaktivität (Details): Anti-Phospho-Ser421 MeCP2 Antibody is directed against MeCP2 protein phosphorylated at S421.

Aufreinigung: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Anti-Phospho-Ser421 MeCP2 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 of mouse MeCP2 protein conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Phospho-Ser421 MeCP2 Antibody is tested for Western Blots and is specific for the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling of the MeCP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Haltbarkeit: 12 months

Referenzen für anti-MECP2 Antikörper (ABIN6658009)

Wang, Tian, Liu, Goldstein, Bado, Zhang, Arenkiel, Li, Yang, Du, Zhao, Rowley, Wong, Gugala, Zhang: "The Osteogenic Niche Is a Calcium Reservoir of Bone Micrometastases and Confers Unexpected Therapeutic Vulnerability." in: Cancer cell, Vol. 34, Issue 5, pp. 823-839.e7, (2019) (PubMed).

Details zu MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Andere Bezeichnung: MeCP2

Hintergrund:

Synonyms: Methyl-CpG-binding protein 2, MeCp-2 protein

Background: MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Defects in MeCP2 are the cause of Rett syndrome (RTT). RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment. MeCP2 pS421 Antibody is ideal for researchers interested in neuroscience research.

Gene Name: MECP2

Gen-ID: 17257

NCBI Accession: NP_001075448

UniProt: Q9Z2D6

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane