STAT4 Antikörper (AA 730-749)

Produktnummer ABIN6657793

Hersteller Produkt- Nr.: 100-401-861

Der Kaninchen Polyklonal Anti-STAT4-Antikörper wurde für WB, ELISA und IP validiert. Er ist geeignet, STAT4 in Proben von Maus zu detektieren.

Kurzübersicht für STAT4 Antikörper (AA 730-749) (ABIN6657793)

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser STAT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-STAT4 Antikörper

Bindungsspezifität: AA 730-749

Hersteller Produkt- Nr.: 100-401-861

Hersteller: Rockland

Verwendungszweck: Stat4 Antibody

Kreuzreaktivität (Details):  Reactivity occurs against mouse STAT4 protein.

Aufreinigung: This antibody was prepared from whole rabbit antiserum by delipidation and defibrination.

Sterilität: Sterile filtered

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 725-749 of Mouse STAT-4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: Use 5 μL

ELISA_Dilution: 1:5,000 - 1:25,000

Western_Blot_Dilution: 1:5,000

Other: User Optimized

Kommentare:

This antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  This product was assayed by western blotting after immunoprecipitation of mouse STAT4 from a lysate containing over expressed protein. A 1:5,000 dilution shows reactivity with a band at 85 kDa corresponding to mouse STAT4. Use 5 μL for immunoprecipitation. A HeLa whole cell lysate or mouse testis lysate are suitable for use as a positive control. Reactivity in other immunoassays is unknown. 

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu STAT4

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Andere Bezeichnung: STAT4

Hintergrund:

Synonyms: rabbit anti-STAT4 Antibody, Signal transducer and activator of transcription 4, Stat4

Background: Signal transducer and activator of transcription 4 (STAT4) carries out a dual function of signal transduction and activation of transcription.  STAT4 forms a homodimer or a heterodimer with related transcription factor STAT family members.  STAT4 has a cytoplasmic localization and is translocated into the nucleus in response to activation by JAK, which phosphorylates STAT4 at a tyrosine residue. Various ligands, including interferons and growth factors, induce activation of the JAK kinases, which induce STAT activation. IL-12 has also been identified as an activator of STAT4. Serine phosphorylation is also required for maximal transcriptional activity. The highest level of STAT4 expression is observed in testis and in myeloid cells.

Gene Name: STAT4

Gen-ID: 20849

UniProt: P42228

Pathways: JAK-STAT Signalweg