CD25 Antikörper (PE)

Produktnummer ABIN6657779

Hersteller Produkt- Nr.: 200-508-n82

Der PE-konjugierte Ratte Monoklonal anti-CD25 Antikörper (Klon 3C7) (ABIN6657779) detektiert spezifisch CD25 in FACS und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für CD25 Antikörper (PE) (ABIN6657779)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD25 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP)

Klon: 3C7

Produktdetails anti-CD25 Antikörper (PE)

Hersteller Produkt- Nr.: 200-508-n82

Hersteller: Rockland

Verwendungszweck: CD25 Phycoerythrin Antibody

Kreuzreaktivität (Details): Cross-reactivity with CD25 from other sources has not been tested.

Aufreinigung: Phycoerythrin conjugated CD25 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD25.

Sterilität: Sterile filtered

Immunogen: Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with IL-2-dependent BALB/c mouse helper T-cell clone HT-2.

Isotyp: IgG2b kappa

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Kommentare:

Anti-CD25 is tested for Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Haltbarkeit: 6 months

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: CD25

Hintergrund:

Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, CD25, Il2ra, Il2r

Background: CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Gene Name: Il2r

Gen-ID: 16184

NCBI Accession: NP_032393

UniProt: P01590

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation