CD25 Antikörper (APC)

Produktnummer ABIN6657775

Hersteller Produkt- Nr.: 200-526-m73

Der Ratte Monoklonal Anti-CD25-Antikörper wurde für FACS validiert. Er ist geeignet, CD25 in Proben von Maus zu detektieren.

Kurzübersicht für CD25 Antikörper (APC) (ABIN6657775)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD25 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Klon: PC61

Produktdetails anti-CD25 Antikörper (APC)

Hersteller Produkt- Nr.: 200-526-m73

Hersteller: Rockland

Verwendungszweck: CD25 APC Antibody

Kreuzreaktivität (Details): Reactivity is observed against mouse CD25.

Aufreinigung: Allophycocyanin conjugated CD25 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against mouse CD25.

Sterilität: Sterile filtered

Immunogen: Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with IL-2-dependent cytolytic mouse T-cell clone B6.1.

Isotyp: IgG1 lambda

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise: Optional[Neutralization_Dilution]: 0.1-0.5 μg/1x10^6 cells

Kommentare:

Anti-CD25 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Haltbarkeit: 6 months

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: CD25

Hintergrund:

Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25 Antibody

Background: CD25 is a 55KDa transmembrane glycoprotein consisting of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. It is expressed during early stages of thymocytes development and in activated CD4+ memory T-lymphocytes. CD25 heterodimerizes with IL2R-beta and IL-2R gamma and forms the tripartite high affinity IL-2 receptor complex. It increases the specificity and affinity of IL-2 receptor complex for IL-2 ligand and is necessary for receptor clustering required for augmentation of IL-2 signaling. Mutations in CD25 result in severe immunodeficiency.

Gene Name: IL2RA

Gen-ID: 16184

NCBI Accession: NP_032393

UniProt: P01590

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation