Dystrophin Antikörper

Produktnummer ABIN6657490

Hersteller Produkt- Nr.: 200-301-p36

Der Maus Monoklonal Anti-Dystrophin-Antikörper wurde für IHC, WB und ELISA validiert. Er ist geeignet, Dystrophin in Proben von Human zu detektieren.

Kurzübersicht für Dystrophin Antikörper (ABIN6657490)

Target: Dystrophin (DMD)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Dystrophin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Klon: MANDYS8

Produktdetails anti-Dystrophin Antikörper

Hersteller Produkt- Nr.: 200-301-p36

Hersteller: Rockland

Verwendungszweck: Dystrophin Antibody

Kreuzreaktivität (Details): This product reacts with human, mouse, rat, and rabbit.

Aufreinigung: Anti-Dystrophin antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Dystrophin antibody was produced in mice by repeated immunizations with recombinant protein corresponding to human dystrophin fragment.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:20,000

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 1-2 μg/mL

Kommentare:

Anti-Dystrophin is tested for Immunohistochemistry -F and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~425 kDa corresponding to the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 200μL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Dystrophin

Target: Dystrophin (DMD)

Andere Bezeichnung: Dystrophin

Hintergrund:

Synonyms: BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272

Background: Dystrophin(DMD) gene has 79 exons spanning at least 2,300 kb (2.3 Mb). The C terminus of the dystrophin protein is encoded by a highly conserved, alternatively spliced region of the gene. beta-dystroglycan binding activity is expressed by the dystrophin fragment spanning amino acids 3026-3345 containing the ZZ domain. DMD transcript is formed by at least 60 exons, the first half of the transcript is formed by a minimum of 33 exons spanning nearly 1000 kb, and the remaining portion has at least 27 exons that may spread over a similar distance. Dystrophin gene is expressed at a higher level in primary cultures of neuronal cells than in astro-glial cells derived from adult mouse brain. overexpression of dystrophin prevents the development of the abnormal mechanical properties associated with dystrophic muscle without causing deleterious side effects. This antibody is suitable for researchers interested in Muscular Dystrophies and Dystrophin-Glycoprotein Complexes and cardiovascular research.

Gene Name: DMD

Gen-ID: 1756

NCBI Accession: NP_000100

UniProt: P11532

Pathways: Skeletal Muscle Fiber Development