Caspase 1 Antikörper (C-Term)

Produktnummer ABIN6655699

Hersteller Produkt- Nr.: 200-301-h62

Der Maus Monoklonal Anti-Caspase 1-Antikörper wurde für WB, IHC und IF validiert. Er ist geeignet, Caspase 1 in Proben von Human zu detektieren.

Kurzübersicht für Caspase 1 Antikörper (C-Term) (ABIN6655699)

Target: Caspase 1 (CASP1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Caspase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: 14F468

Produktdetails anti-Caspase 1 Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 200-301-h62

Hersteller: Rockland

Verwendungszweck: Caspase-1 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Caspase-1 from human and mouse based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Caspase-1 Antibody was purified by Protein G chromatography.

Immunogen: Caspase-1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids near the c-terminus of the immunogen, human Caspase-1.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 1:100 - 1:500

Western_Blot_Dilution: 0.5-2 μg/mL

Kommentare:

Anti-Caspase-1 antibody has been tested in IHC-F, IHC-P, IF, and WB. Expect a band approximately 46kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Caspase 1

Target: Caspase 1 (CASP1)

Andere Bezeichnung: Caspase-1

Hintergrund:

Synonyms: IL1BC, IL1BCE, Caspase-1, CASP-1, 3.4.22.36, Interleukin-1 beta convertase, IL-1BC, Interleukin-1 beta-converting enzyme, ICE, IL-1 beta-converting enzyme, p45, Caspase-1 subunit p20, Caspase-1 subunit p10

Background: Anti-Caspase-1 detects human Caspase. Caspase proteins are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspase proteins is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into tetramer to form the active enzyme. Caspase-1 (ICE, IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor. Anti-Caspase-1 antibody is ideal for investigators involved in apoptosis research.

Gene Name: CASP1

Gen-ID: 834

NCBI Accession: NP_001214

UniProt: P29466

Pathways: Apoptose, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome