Caspase 3 Antikörper

Produktnummer ABIN6655470

Hersteller Produkt- Nr.: 200-301-h63

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Caspase 3 in WB, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Caspase 3 Antikörper (ABIN6655470)

Target: Caspase 3 (CASP3)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Caspase 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 31A1067

Produktdetails anti-Caspase 3 Antikörper

Hersteller Produkt- Nr.: 200-301-h63

Hersteller: Rockland

Verwendungszweck: Caspase-3 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Caspase-3 from Human, mouse and rat based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Caspase-3 Antibody was purified by Protein G chromatography.

Immunogen: Caspase-3 Antibody was produced in mice by repeated immunizations with a full length recombinant Caspase-3 human protein.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 5 μg/mL

Western_Blot_Dilution: 1-5 μg/mL

Kommentare:

Anti-Caspase 3 Antibody is tested for use in WB, EM, Flow, ICC/IF, IHC-Fr, and IHC-P. Expect a band approximately 31.5 kDa on specific lysates. The antibody detects both pro Caspase-3(32 kDa) and the large subunit of the active/cleaved form (14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Caspase 3 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Caspase 3

Target: Caspase 3 (CASP3)

Andere Bezeichnung: Caspase-3

Hintergrund:

Synonyms: Cpp32, Caspase-3 subunit p17, Caspase-3 subunit p12, Apopain, Cysteine protease CPP32 Caspase-3, CASP-3, 3.4.22.56, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12

Background: Anti-Caspase-3 antibody detects human Caspase-3. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32- kDa protein. Caspase-3 cleaves the death substrate poly (ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17- kDa and a small subunit of 12- kDa. Anti-Caapase-3 is ideal for investigators involved in apoptosis, cytokines and growth factor research.

Gene Name: Casp3

Gen-ID: 836

NCBI Accession: NP_004337

UniProt: P42574

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation