CD25 Antikörper (FITC)

Produktnummer ABIN6655112

Produktdetails anti-CD25 Antikörper (FITC)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD25 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Kreuzreaktivität (Details): Cross reactivity with CD25 from other sources has not been tested

Aufreinigung: Fluorescein conjugated CD25 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD25. Reactivity is observed against human CD25, Baboon, Chimpanzee, Cynomolgus, Rhesus, and Pigtailed Macaque. . Anti-CD25 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Immunogen:

Immunogen: Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with CD25 antigen.

Klon: BC96

Isotyp: IgG1

Labeling Ratio: 4-6

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-CD25 is useful for Flow Cytometry and Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

Flow Cytometry Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Referenzen für anti-CD25 Antikörper (FITC) (ABIN6655112)

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, (1993) (PubMed).

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, (1991) (PubMed).

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: CD25 (IL2RA Produkte)

Synonyme: CD25 antikoerper, IDDM10 antikoerper, IL2R antikoerper, TCGFR antikoerper, IL2RAC antikoerper, IL-2RA antikoerper, Il2r antikoerper, Ly-43 antikoerper, interleukin 2 receptor subunit alpha antikoerper, interleukin-2 receptor subunit alpha-like antikoerper, interleukin 2 receptor, alpha chain antikoerper, IL2RA antikoerper, Il2ra antikoerper, LOC100222957 antikoerper

Hintergrund:

Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55

Background: CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Gene Name: IL2RA

Gen-ID: 3559

NCBI Accession: NP_000408

UniProt: P01589

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation