ATF2 Antikörper (pSer490, pSer498)

Produktnummer ABIN6655069

Hersteller Produkt- Nr.: 609-401-d11

Der Kaninchen Polyklonal anti-ATF2 Antikörper wird verwendet zum Nachweis von ATF2 in Proben von Human. Er wurde validiert für WB, IHC und ELISA.

Kurzübersicht für ATF2 Antikörper (pSer490, pSer498) (ABIN6655069)

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-ATF2 Antikörper

Bindungsspezifität: pSer490, pSer498

Hersteller Produkt- Nr.: 609-401-d11

Hersteller: Rockland

Verwendungszweck: ATF2 phospho S490/phospho S498 Antibody

Kreuzreaktivität (Details): Anti-ATF2 pS490/pS498 Antibody is directed against ATF2 protein phosphorylated at S490/pS498.

Aufreinigung: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-ATF2 pS490/pS498 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser490/498.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-ATF2 pS490/pS498 antibody is tested for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. ATF2 antibody is specific for the approximately 74kDa ATF2 protein phosphorylated at Ser490,498. Anti-ATF2 also recognizes the phosphorylated 54kDa splice form of ATF2.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu ATF2

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Andere Bezeichnung: ATF2

Hintergrund:

Synonyms: rabbit anti-ATF2 pS490/pS498 Antibody, Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, CREB-2, HB16, cAMP response element-binding protein CRE-BP1

Background: ATF2 antibody recognizes the activating transcription factor ATF2 (also called CRE-BP1) which binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2. Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF. Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered an important substrate of signals upstream of the activation of genes associated with neuronal growth and differentiation. ATF expression has also been linked to the depression in humans. Anti-ATF2 pS490/pS498 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: ATF2

Gen-ID: 1386

UniProt: P15336

Pathways: MAPK Signalweg, RTK Signalweg, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades