Ubiquitin B Antikörper

Produktnummer ABIN6655045

Hersteller Produkt- Nr.: 200-301-g61

Produktdetails anti-Ubiquitin B Antikörper

Target: Ubiquitin B (UBB)

Reaktivität: Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ubiquitin B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Hersteller Produkt- Nr.: 200-301-g61

Hersteller: Rockland

Verwendungszweck: Ubiquitin Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with ubiquitin from Human, mouse, rat, and bovine based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Ubiquitin Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Ubiquitin Antibody was produced in mice by repeated immunizations raised against native bovine ubiquitin conjugated to KLH.

Klon: 5B9-B3

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:200

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Ubiquitin Antibody is tested for use in WB and IF. Expect a band approximately ~10kDa corresponding to free ubiquitin. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Ubiquitin B

Target: Ubiquitin B (UBB)

Andere Bezeichnung: UBB (UBB Produkte)

Hintergrund:

Synonyms: Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B

Background: Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96 % sequence identity. The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate. Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkBα and p27.

Gene Name: UBB

Gen-ID: 281370

NCBI Accession: NP_776558

UniProt: P0CG53

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagie, EGFR Downregulation, Ubiquitin Proteasome Pathway