GRP94 Antikörper

Produktnummer ABIN6655034

Hersteller Produkt- Nr.: 200-501-f39

Dieses Anti-GRP94-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von GRP94 in WB, FACS, IF und IP. Geeignet für Huhn.

Kurzübersicht für GRP94 Antikörper (ABIN6655034)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reaktivität: Huhn

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser GRP94 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 9G10

Produktdetails anti-GRP94 Antikörper

Hersteller Produkt- Nr.: 200-501-f39

Hersteller: Rockland

Verwendungszweck: GRP94 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with GRP94 from Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea pig, Hamster, Horse, Monkey, Pig, Rabbit, Sheep, Xenopus sources based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-GRP94 Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Grp94 Antibody was produced in rat by repeated immunizations with purified Grp94 isolated from chicken oviducts.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: User Optimized

Western_Blot_Dilution: 0.5 μg/mL

Kommentare:

Anti-Grp94 Antibody is tested for WB, IF, IP, and Flow cytometry. Expect a band approximately 98kDa protein corresponding to the molecular mass of Grp94 on SDS PAGE immunoblots. This monoclonal antibody reacts with purified Grp94. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Andere Bezeichnung: GRP94

Hintergrund:

Synonyms: HSP90B1, GP96, TRA1, ECGP, Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog

Background: Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion. Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homolgy with its cytosolic equivalent, Hsp90. Both Hsp90 and Grp94 are calcium binding proteins. Despite sharing 50 % sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity. Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER

Gene Name: HSP90B1

Gen-ID: 374163

NCBI Accession: NP_989620

UniProt: P08110

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades