PCNA Antikörper

Produktnummer ABIN6297605

Der Maus Monoklonal Anti-PCNA-Antikörper wurde für WB und IHC validiert. Er ist geeignet, PCNA in Proben von Human zu detektieren.

Kurzübersicht für PCNA Antikörper (ABIN6297605)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-PCNA Antikörper

Verwendungszweck: Mouse anti-Human PCNA Antibody [Sodium Azide Free]

Spezifität: Nuclear with some cytoplasmic

Aufreinigung: Recombinant proliferating cell nuclear antigen was used as the immunogen for the PCNA antibody.

Immunogen: Recombinant proliferating cell nuclear antigen was used as the immunogen for the PCNA antibody.

Anwendungsinformationen

Applikationshinweise: Western blot: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: Proliferating cell nuclear antigen

Hintergrund:

Target Description: Proliferating cell nuclear antigen is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. [UniProt]

Gene Symbol: PCNA

Gen-ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie