Keratin 77 Antikörper

Produktnummer ABIN6296409

Dieses Anti-Keratin 77-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Keratin 77 in IHC, WB, IF und FACS. Geeignet für Human, Ratte, Rind (Kuh), Hund, Maus, Kaninchen, Huhn und Affe.

Kurzübersicht für Keratin 77 Antikörper (ABIN6296409)

Target: Keratin 77 (KRT77)

Reaktivität: Human, Ratte, Rind (Kuh), Hund, Maus, Kaninchen, Huhn, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 77 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-Keratin 77 Antikörper

Verwendungszweck: Mouse anti-Human/Monkey/Cow/Dog/Rabbit/Mouse/Rat/Chicken pan Cytokeratin Antibody Cocktail [Sodium Azide Free]

Spezifität: Cytoplasmic

Aufreinigung: Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.

Immunogen: Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu Keratin 77

Target: Keratin 77 (KRT77)

Andere Bezeichnung: Keratin, type II cytoskeletal 1b

Hintergrund:

Target Description: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 55 kDa (CK7), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 46 kDa (CK17), 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.

Gene Symbol: KRT77///KRT1B///KRT76///KRT2B///KRT2P

UniProt: Q7Z794, Q01546