GJB1 Antikörper (Cytoplasmic Loop)

Produktnummer ABIN6295447

Dieses Maus Monoklonal-Antikörper erkennt spezifisch GJB1 in WB, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für GJB1 Antikörper (Cytoplasmic Loop) (ABIN6295447)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GJB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-GJB1 Antikörper

Bindungsspezifität: Cytoplasmic Loop

Verwendungszweck: Mouse anti-Rat/Mouse/Human Connexin 32 Antibody / GJB1 [Sodium Azide Free]

Spezifität: Cell surface, cytoplasmic

Aufreinigung: Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).

Immunogen: Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (Frozen): 0.5-1 μg/mL for 30 min at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Andere Bezeichnung: Gap junction beta-1 protein

Hintergrund:

Target Description: This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.

Gene Symbol: GJB1///CX32

Gen-ID: 2705

UniProt: P08034

Pathways: Cell-Cell Junction Organization