RECQL2 Antikörper (Internal Region)

Produktnummer ABIN6266052

Der Kaninchen Polyklonal Anti-RECQL2-Antikörper wurde für WB, ELISA, IHC, IF und ICC validiert. Er ist geeignet, RECQL2 in Proben von Human und Maus zu detektieren.

Kurzübersicht für RECQL2 Antikörper (Internal Region) (ABIN6266052)

Target: RECQL2 (WRN) (Werner syndrome RecQ like helicase (WRN))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RECQL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-RECQL2 Antikörper

Bindungsspezifität: Internal Region

Spezifität: WRN Antibody detects endogenous levels of total WRN.

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human WRN, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu RECQL2

Target: RECQL2 (WRN) (Werner syndrome RecQ like helicase (WRN))

Andere Bezeichnung: WRN

Hintergrund:

Description: Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers, stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation.

Gene: WRN

Molekulargewicht: 162 kDa

Gen-ID: 7486

UniProt: Q14191

Pathways: DNA Reparatur