PKM2 Antikörper (Internal Region)

Produktnummer ABIN6264260

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PKM2 in WB, IHC, ICC und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.Es wurde in 1 Publikation zitiert.

Kurzübersicht für PKM2 Antikörper (Internal Region) (ABIN6264260)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-PKM2 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: PKM2 Antibody

Spezifität: PKM2 Antibody detects endogenous levels of total PKM2.

Homologie: Pig,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PKM2, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Referenzen für anti-PKM2 Antikörper (ABIN6264260)

Xie, Huang, Tang, Ye, Yang, Guo, Tian, Xie, Peng, Xie: "Diallyl Disulfide Inhibits Breast Cancer Stem Cell Progression and Glucose Metabolism by Targeting CD44/PKM2/AMPK Signaling." in: Current cancer drug targets, Vol. 18, Issue 6, pp. 592-599, (2018) (PubMed).

Details zu PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Andere Bezeichnung: PKM2

Hintergrund:

Description: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Gene: PKM

Molekulargewicht: 58 kDa

Gen-ID: 5315

UniProt: P14618

Pathways: Warburg Effekt