ATG5 Antikörper (C-Term)

Produktnummer ABIN6260107

Dieses Anti-ATG5-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ATG5 in WB, IHC und ELISA. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für ATG5 Antikörper (C-Term) (ABIN6260107)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-ATG5 Antikörper

Bindungsspezifität: C-Term

Spezifität: APG5L/ATG5 Antibody detects endogenous levels of total APG5L/ATG5.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human APG5L/ATG5, corresponding to a region within C-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Referenzen für anti-ATG5 Antikörper (ABIN6260107)

Li, Li, Zeng, Yang, Liu, Zhang, Song, Yao, Ma, Li, Wang, Wei: "Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis." in: Journal of virology, Vol. 92, Issue 8, (2018) (PubMed).

Details zu ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Andere Bezeichnung: ATG5

Hintergrund:

Description: Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.

Gene: ATG5

Molekulargewicht: 32kDa

Gen-ID: 9474

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagie