RAD51 Homolog B Antikörper

Produktnummer ABIN6259179

Der Kaninchen Polyklonal Anti-RAD51 Homolog B-Antikörper wurde für WB, ELISA, IHC, IF und ICC validiert. Er ist geeignet, RAD51 Homolog B in Proben von Human, Maus und Affe zu detektieren.

Kurzübersicht für RAD51 Homolog B Antikörper (ABIN6259179)

Target: RAD51 Homolog B (Rad51B)

Reaktivität: Human, Maus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD51 Homolog B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-RAD51 Homolog B Antikörper

Spezifität: RAD51L1 Antibody detects endogenous levels of total RAD51L1

Kreuzreaktivität: Human, Affe, Maus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500~1:1000 IHC: 1:50~1:200, IF/ICC 1:100-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C.Stable for 12 months from date of receipt

Haltbarkeit: 12 months

Details zu RAD51 Homolog B

Target: RAD51 Homolog B (Rad51B)

Andere Bezeichnung: RAD51L1

Hintergrund:

Description: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway.

Gene: RAD51B

Molekulargewicht: 45 kDa

Gen-ID: 5890

UniProt: O15315

Pathways: DNA Reparatur