GATA4 Antikörper (pSer262)

Produktnummer ABIN6256227

Der Kaninchen Polyklonal Anti-GATA4-Antikörper wurde für WB, ELISA, IHC, IF und ICC validiert. Er ist geeignet, GATA4 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für GATA4 Antikörper (pSer262) (ABIN6256227)

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GATA4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-GATA4 Antikörper

Bindungsspezifität: pSer262

Spezifität: Phospho-GATA4 (Ser262) Antibody detects endogenous levels of GATA4 only when phosphorylated at Serine 262.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human GATA4 around the phosphorylation site of Ser262.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu GATA4

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Andere Bezeichnung: GATA4

Hintergrund:

Description: Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function (PubMed:24000169, PubMed:27984724). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (PubMed:27984724). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).

Gene: GATA4

Molekulargewicht: 48kDa

Gen-ID: 2626

UniProt: P43694

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis