GATA4 Antikörper (pSer262)

Produktnummer ABIN6256227

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-GATA4 Antikörper wird verwendet zum Nachweis von GATA4 in Proben von Human, Ratte und Maus. Er wurde validiert für WB, ELISA, IF, IHC und ICC.

Kurzübersicht für GATA4 Antikörper (pSer262) (ABIN6256227)

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GATA4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Produktdetails anti-GATA4 Antikörper

Bindungsspezifität: pSer262

Spezifität: Phospho-GATA4 (Ser262) Antibody detects endogenous levels of GATA4 only when phosphorylated at Serine 262.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human GATA4 around the phosphorylation site of Ser262.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Referenzen für anti-GATA4 Antikörper (ABIN6256227)

Karimi, Jalili, Mansouri, Bahremand, Gholami: "Effect of melatonin on steroidogenesis-related enzymes expression and testosterone synthesis following CoCl2-induced hypoxia in TM3 Leydig cells." in: Iranian journal of basic medical sciences, Vol. 26, Issue 9, pp. 1041-1046, (2023) (PubMed).

Details zu GATA4

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Andere Bezeichnung: GATA4

Hintergrund:

Description: Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function (PubMed:24000169, PubMed:27984724). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (PubMed:27984724). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).

Gene: GATA4

Molekulargewicht: 48kDa

Gen-ID: 2626

UniProt: P43694

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis