GFM1 Antikörper

Produktnummer ABIN6252740

Dieses Anti-GFM1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von GFM1 in WB, ELISA, IF und IHC (p). Geeignet für Human.

Kurzübersicht für GFM1 Antikörper (ABIN6252740)

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: AE-1

Produktdetails anti-GFM1 Antikörper

Spezifität: Does not react with mouse and rat

Aufreinigung: Protein G affinity chromatography

Immunogen: A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondrial antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: ELISA: order Ab without BSA

Immunofluorescence: 0.5-1 μg/mL

Western blot: 0.25-0.5 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the anti-Mitochondrial antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu GFM1

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Andere Bezeichnung: Mitochondrial

Hintergrund: This mAb recognizes a 60  kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.