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GFM1 Antikörper

GFM1 Reaktivität: Human WB, IF, IHC (p), FACS Wirt: Maus Monoclonal MTC719 unconjugated
Produktnummer ABIN3025203
  • Target Alle GFM1 Antikörper anzeigen
    GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))
    Reaktivität
    • 23
    • 18
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 28
    • 6
    Maus
    Klonalität
    • 28
    • 6
    Monoklonal
    Konjugat
    • 20
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser GFM1 Antikörper ist unkonjugiert
    Applikation
    • 33
    • 13
    • 13
    • 10
    • 10
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
    Keine Kreuzreaktivität
    Insekten, Bakterien
    Produktmerkmale
    MAb MTC719 recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC719 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondrial antibody.
    Klon
    MTC719
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product GFM1 Primärantikörper
  • Applikationshinweise
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Mitochondrial antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.25-0.5 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Konservierungsmittel
    Azide free
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store the Mitochondrial antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))
    Andere Bezeichnung
    Mitochondrial (GFM1 Produkte)
    Synonyme
    COXPD1 antikoerper, EFG antikoerper, EFG1 antikoerper, EFGM antikoerper, EGF1 antikoerper, GFM antikoerper, hEFG1 antikoerper, AW545374 antikoerper, D3Wsu133e antikoerper, Gfm antikoerper, EF-G antikoerper, Efg antikoerper, G elongation factor mitochondrial 1 antikoerper, G elongation factor, mitochondrial 1 antikoerper, GFM1 antikoerper, Gfm1 antikoerper
    Hintergrund
    MAb MTC719 recognizes a 60  kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC719 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
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