GJB1 Antikörper

Produktnummer ABIN6252465

Dieser Ratte Monoklonal Antikörper detektiert spezifisch GJB1 in WB, FACS, IF und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Ratte und Maus.

Kurzübersicht für GJB1 Antikörper (ABIN6252465)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reaktivität: Ratte, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser GJB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: R5-21C

Produktdetails anti-GJB1 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Mouse liver DOC-JR-plasma membranes were used as the immunogen for the Connexin 32 antibody.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

WB: 0.5-1 μg/mL

IHC (Frozen): 1-2 μg/mL for 30 min at RT

Optimal dilution of the Connexin 32 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Andere Bezeichnung: Connexin 32

Hintergrund: This Ab recognizes a protein of 27-32  kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.

Gen-ID: 14618

UniProt: P28230

Pathways: Cell-Cell Junction Organization