PCNA Antikörper

Produktnummer ABIN6252102

Dieser Maus Monoklonal Antikörper detektiert spezifisch PCNA in WB, IHC, FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Huhn, Schwein, Affe, Saccharomyces cerevisiae, Zebrafisch (Danio rerio) und Drosophila melanogaster.

Kurzübersicht für PCNA Antikörper (ABIN6252102)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Maus, Ratte, Huhn, Schwein, Affe, Saccharomyces cerevisiae, Zebrafisch (Danio rerio), Drosophila melanogaster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: SPM350

Produktdetails anti-PCNA Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Rat PCNA protein was used as the immunogen for this anti-PCNA antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Western blot: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the anti-PCNA antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Hintergrund: Recognizes a non-histone protein of 36  kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.

Gen-ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie