ATF6 Antikörper (N-Term)

Produktnummer ABIN6251795

Der Maus Monoklonal anti-ATF6 Antikörper (Klon AF6229) (ABIN6251795) detektiert spezifisch ATF6 in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für ATF6 Antikörper (N-Term) (ABIN6251795)

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATF6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: AF6229

Produktdetails anti-ATF6 Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Protein G purified monoclonal antibody

Immunogen: A recombinant protein fragment from the N-terminal region of human ATF6 was used as the immunogen for this antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Western blot: 2-4 μg/mL (2)

IHC (Paraffin): 5 μg/mL (1)

ATF6 is a 670 amino acid protein that migrates in western blot between 70~90 kD due to glycosylation.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. ATF6 is seen in western blotting between 75~90KD.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Andere Bezeichnung: ATF6

Hintergrund: ER stress can cause the accumulation of unfolded proteins and lead to the activation of the UPR (unfolded proetin response). Cells have developed an intracellular signaling pathway to deal with this stress, and Activating Transcription Factor 6 (ATF6) is a major transducer of the UPR. Upon activation, ATF6 seperates from GPR78 and is cleaved. The ~50 kD cytosolic portion of ATF6 translocates to the nucleus, transmitting the ER stress signal. In the nucleus, ATF6 activates the transcription of UPR-responsive genes by biniding to their ERSE (ER stress response element) region.

Gen-ID: 22926

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response