ATF6 Antikörper (N-Term)

Produktnummer ABIN6655678

Produktdetails anti-ATF6 Antikörper

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATF6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Kreuzreaktivität: Human, Maus

Aufreinigung: Anti-ATF6 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-ATF6 from Grouper (Fish), Hamster, Human, Mouse, Rabbit, and Rat based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-ATF6 from other sources has not been determined.

Immunogen:

Immunogen: ATF6 Antibody was produced in mice by repeated immunizations with the partial protein of human ATF6 at the n-terminus of the protein.

Immunogen Type: Peptide

Klon: 70B1413-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-ATF6 antibody is suitable for use in ChIP, IF/ICC, IHC (frozen), IHC (p), IP and WB. Expect a band approximately 90 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1-5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Details zu ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Andere Bezeichnung: ATF6 (ATF6 Produkte)

Synonyme: ATF-6-like antikoerper, GB16435 antikoerper, ATF6 antikoerper, Atf6 antikoerper, ATF6A antikoerper, 9130025P16Rik antikoerper, 9630036G24 antikoerper, AA789574 antikoerper, Atf6alpha antikoerper, ESTM49 antikoerper, activating transcription factor 6 antikoerper, uncharacterized LOC412432 antikoerper, activating transcription factor 6 S homeolog antikoerper, cyclic AMP-dependent transcription factor ATF-6 alpha antikoerper, ATF6 antikoerper, LOC412432 antikoerper, atf6.S antikoerper, LOC100122162 antikoerper, Atf6 antikoerper

Hintergrund:

Synonyms: Activating transcription factor 6 alpha

Background: Anti-ATF6 antibody detects human ATF6. ATF6 is a constitutively expressed, endoplasmic reticulum (ER) membrane-anchored transcription factor. ATF6 is a key transcriptional activator of the unfolded protein response (UPR), which allows mammalian cells to maintain cellular homeostasis when they are subjected to environmental and physiological stresses that target the ER. The C-terminus of ATF6 is located in the ER lumen and its N-terminal DNA binding domain faces the cytosol. AFT6 plays a key role in the ER stress response by transmitting the ER stress signal across the ER membrane into the nucleus. The induction of new gene expression by ATF6 is an important aspect of the ER stress response. In response to certain stress conditions, ATF6 translocates from the ER to the Golgi. The 90 kDa full-length ATF6 is processed within the Golgi to its active 50 kDa form through sequential cleavage by site-1 and site-2 proteases (S1P and S2P). Proteolytic activation of ATF6 in the ER stress response is a mechanism to regulate membrane-bound factors, and is referred to as regulated intramembrane proteolysis. The N-terminal active ATF6 translocates to the nucleus where it binds to ER stress-response elements in ER stress-response genes (ERSRGs). ATF6 is a potent transcriptional activator of ERSRGs. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment. Anti-ATF6 antibody is ideal for investigators involved in apoptosis research.

Gene Name: ATF6

Gen-ID: 22926

NCBI Accession: NP_031374

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response