ATF6 Antikörper (N-Term)

Produktnummer ABIN6655071

Produktdetails anti-ATF6 Antikörper

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATF6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Aufreinigung: This affinity purified antibody is directed against human ATF6. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human and 80% identity to rat.

Immunogen:

Immunogen: Anti-ATF6 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Term portion of human ATF6 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200

Application Note: Anti-ATF6 Antibody has been tested in Western Blot, IF and ELISA. Recombinant ATF6 protein positive control was used for western blotting. Expect a band at 74.5 kDa in western blot using appropriate lysates or recombinant protein. Antibody tested positive in U251 cells (W09-001-GY4) in Immunofluorescence. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 5.0 μg/mL

Flow Cytometry Dilution: User Optimized

Western Blot Dilution: 0.5 μg/mL

IF Microscopy Dilution: 10 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Details zu ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Andere Bezeichnung: ATF6 (ATF6 Produkte)

Synonyme: ATF-6-like antikoerper, GB16435 antikoerper, ATF6 antikoerper, Atf6 antikoerper, ATF6A antikoerper, 9130025P16Rik antikoerper, 9630036G24 antikoerper, AA789574 antikoerper, Atf6alpha antikoerper, ESTM49 antikoerper, activating transcription factor 6 antikoerper, uncharacterized LOC412432 antikoerper, activating transcription factor 6 S homeolog antikoerper, cyclic AMP-dependent transcription factor ATF-6 alpha antikoerper, ATF6 antikoerper, LOC412432 antikoerper, atf6.S antikoerper, LOC100122162 antikoerper, Atf6 antikoerper

Hintergrund:

Synonyms: rabbit anti-ATF6 Antibody, Activating Transcription Factor 6, rabbit anti-ATF-6 Antibody, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, ATF6 alpha

Background: ATF6 belongs to the bZIP (basic-leucine zipper domain transcription factor) family. Activating target genes for the unfolded protein response during endoplasmic reticulum stress. Embeded in the endoplasmic reticulum, it functions as an ER stress sensor/stress transducer. Following stress-induced proteolysis it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. Binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in the activation of transcription by the serum response factor. This protein may play a role in the development of the foveal and cone function in the retina. Therefore, attributing to disorders such as achromatopsia, blindness, nystagmus, myopia, cataracts and more. Anti-ATF6 Antibody is useful for researchers studying eye development, protein folding and processing, Endoplasmic Reticulum Stress Pathways, Glucose and Energy Metabolism Research.

Gene Name: ATF6

Gen-ID: 22926

NCBI Accession: NP_031374

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response