WEE1 Antikörper (Ser123)

Produktnummer ABIN6243968

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch WEE1 in WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für WEE1 Antikörper (Ser123) (ABIN6243968)

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WEE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: RB57984

Produktdetails anti-WEE1 Antikörper

Bindungsspezifität: AA 96-130, Ser123

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Wee1(S123) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 96-130 amino acids from human Wee1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Details zu WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Andere Bezeichnung: Wee1

Hintergrund: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Molekulargewicht: 71597

UniProt: P30291

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, M Phase