Ubiquitin B Antikörper (N-Term)

Produktnummer ABIN6242685

Der Kaninchen Polyklonal Anti-Ubiquitin B-Antikörper wurde für WB validiert. Er ist geeignet, Ubiquitin B in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Ubiquitin B Antikörper (N-Term) (ABIN6242685)

Target: Ubiquitin B (UBB)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ubiquitin B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: RB49507

Produktdetails anti-Ubiquitin B Antikörper

Bindungsspezifität: AA 25-57, N-Term

Homologie: B, E, D, Pr, Pig, Sh, C, Hs, Ha, X, Rb

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This UBB(Ubiquitin) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-57amino acids from the N-terminal region of human UBB(Ubiquitin).

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Details zu Ubiquitin B

Target: Ubiquitin B (UBB)

Andere Bezeichnung: UBB(Ubiquitin)

Hintergrund: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair, Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation, Lys-29-linked is involved in lysosomal degradation, Lys-33-linked is involved in kinase modification, Lys-48-linked is involved in protein degradation via the proteasome, Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Molekulargewicht: 25762

UniProt: P0CG47

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagie, EGFR Downregulation, Ubiquitin Proteasome Pathway