GJB1 Antikörper (Biotin)

Produktnummer ABIN6178431

Dieses Maus Monoklonal-Antikörper erkennt spezifisch GJB1 in WB, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für GJB1 Antikörper (Biotin) (ABIN6178431)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GJB1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: GJB1-1753

Produktdetails anti-GJB1 Antikörper (Biotin)

Verwendungszweck: Mouse Monoclonal anti-Connexin-32 (GJB1/1753), Biotin Conjugate

Produktmerkmale: This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane ?-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human GJB1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• Immunofluorescence 1-2 μg/mL Flow cytometry 0.5-1 μg/million cells
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

MCF-7 Cells. Liver, Kidney, Stomach or Tonsil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Andere Bezeichnung: Connexin-32

Hintergrund: Charcot Marie Tooth neuropathy X linked, CMTX, CMTX1, Connexin-32, Cx32, GAP junction 28  kDa liver protein, Gap junction beta-1 protein, Gap junction protein beta 1 32kD, GJB1

Molekulargewicht: 27-32 kDa

Gen-ID: 2705, 333303

UniProt: P08034

Pathways: Cell-Cell Junction Organization