BRCA1 Antikörper (AA 445-620) (CF®647)
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- Target Alle BRCA1 Antikörper anzeigen
- BRCA1 (Breast Cancer 1 (BRCA1))
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Bindungsspezifität
- AA 445-620
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser BRCA1 Antikörper ist konjugiert mit CF®647
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Applikation
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Verwendungszweck
- Mouse Monoclonal anti-BRCA1 (BRCA1/1398), CF647 Conjugate
- Produktmerkmale
- The BRCA1 gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human BRCA1 protein fragment (aa445-620) (exact sequence is proprietary)
- Klon
- BRCA1-1398
- Isotyp
- IgG1
- Top Product
- Discover our top product BRCA1 Primärantikörper
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- Applikationshinweise
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Immunohistology Formalin-fixed 5-10 μg/mL for 60 min at RT
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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A431 or HeLa Cells. Breast, Ovarian or Renal Cell Carcinoma
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- BRCA1 (Breast Cancer 1 (BRCA1))
- Andere Bezeichnung
- BRCA1 (BRCA1 Produkte)
- Synonyme
- BRCAI antikoerper, BRCC1 antikoerper, BROVCA1 antikoerper, IRIS antikoerper, PNCA4 antikoerper, PPP1R53 antikoerper, PSCP antikoerper, RNF53 antikoerper, iris antikoerper, pscp antikoerper, brcai antikoerper, brcc1 antikoerper, rnf53 antikoerper, brca1 antikoerper, BRCA1 antikoerper, LOC100224649 antikoerper, BRCA1, DNA repair associated antikoerper, breast cancer 1 antikoerper, breast cancer 1, early onset antikoerper, breast cancer 1 L homeolog antikoerper, BRCA1 antikoerper, brca1 antikoerper, LOC755086 antikoerper, Brca1 antikoerper, brca1.L antikoerper
- Molekulargewicht
- 220 kDa
- Gen-ID
- 672, 194143
- UniProt
- P38398
- Pathways
- Zellzyklus, DNA Reparatur, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus
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