PCNA Antikörper

Produktnummer ABIN6166258

Der Maus Monoklonal Anti-PCNA-Antikörper wurde für WB, IF, FACS und IHC (f) validiert. Er ist geeignet, PCNA in Proben von Human, Maus, Ratte, Huhn, Affe, Schwein, Saccharomyces cerevisiae, Zebrafisch (Danio rerio), Drosophila melanogaster und Schizosaccharomyces pombe zu detektieren.

Kurzübersicht für PCNA Antikörper (ABIN6166258)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Maus, Ratte, Huhn, Affe, Schwein, Saccharomyces cerevisiae, Zebrafisch (Danio rerio), Drosophila melanogaster, Schizosaccharomyces pombe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: PC10

Produktdetails anti-PCNA Antikörper

Verwendungszweck: Mouse Monoclonal anti-PCNA (PC10), Purified, BSA-free

Produktmerkmale: This antibody recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Rat PCNA/Protein A fusion protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Tonsil or reactive lymph node

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Konservierungsmittel: Azide free

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Hintergrund: Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein

Molekulargewicht: 36 kDa

Gen-ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie