Calnexin Antikörper (N-Term) (CF®488A)

Produktnummer ABIN6161455

Der Maus Monoklonal Anti-Calnexin-Antikörper wurde für WB, IHC, IF, FACS und ISt validiert. Er ist geeignet, Calnexin in Proben von Human zu detektieren.

Kurzübersicht für Calnexin Antikörper (N-Term) (CF®488A) (ABIN6161455)

Target: Calnexin (CANX)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Calnexin Antikörper ist konjugiert mit CF®488A

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt)

Klon: CANX-1541

Produktdetails anti-Calnexin Antikörper (CF®488A)

Bindungsspezifität: N-Term

Verwendungszweck: Mouse Monoclonal anti-Calnexin (CANX/1541), CF488A Conjugate

Produktmerkmale: This antibody recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant N-terminal fragment of human Calnexin protein (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Calnexin

Target: Calnexin (CANX)

Andere Bezeichnung: Calnexin

Hintergrund: Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90

Molekulargewicht: 90 kDa

Gen-ID: 821, 567968

UniProt: P27824

Pathways: MAPK Signalweg, Thyroid Hormone Synthesis