TRIM29 Antikörper (Internal Region)

Produktnummer ABIN571104

Dieses Anti-TRIM29-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von TRIM29 in WB, ELISA, IHC, IF und FACS. Geeignet für Human.

Kurzübersicht für TRIM29 Antikörper (Internal Region) (ABIN571104)

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TRIM29 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-TRIM29 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: TRIM29 / ATDC

Sequenz: EKDRIKSFTT NE

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EKDRIKSFTTNE, from the internal region of the protein sequence according to NP_036233.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 37 kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) and in lysates of cell lines HeLa. An additional 70 kDa band was seen in some PBM lysates (calculated MW of 37.3 kDa according to NP_001317311.1 (isoform 2) and 65.8

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the Cytoplasm and Intermediate filaments of A431 cells. Strong expression of the protein seen in the Nucleus of U2OS cells. Recommended concentration: 10μg/ml.

Flow C

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu TRIM29

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Andere Bezeichnung: TRIM29

Hintergrund: TRIM29, tripartite motif-containing 29, ATDC, FLJ36085, ataxia-telangiectasia group D-associated protein, tripartite motif protein TRIM29

Molekulargewicht: 65.8kDa

Gen-ID: 23650, 72169, 300656

NCBI Accession: NP_036233, NP_001317311

Pathways: p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus