Calnexin Antikörper (Internal Region)

Produktnummer ABIN571025

Der Ziege-Polyklonal Anti-Calnexin-Antikörper wurde für WB, IHC, IF und ELISA validiert. Er ist geeignet zum Nachweis von Calnexin in Proben aus Human und Ratte. }

Kurzübersicht für Calnexin Antikörper (Internal Region) (ABIN571025)

Target: Calnexin (CANX)

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Calnexin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Güteklasse: Verified

Produktdetails anti-Calnexin Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Calnexin

Sequenz: SKTPELNLDQ FHDKT

Spezifität: Reported variants represent identical protein (NP_001019820.1, NP_001737.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SKTPELNLDQFHDKT, from the internal region (near N Terminus) of the protein sequence according to NP_001737.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Cortex. Recommended concentration: 6 μg/mL.

Western Blot: Approx 90-100 kDa band observed in Human Cerebellum and Rat Brain lysates and in lysates of cell lines LNCaP and U251 (calculated MW of 67.6 kDa according to Human NP_001737.1 and 67.3 kDa according to Rat NP_742005.1). This molecular weight i

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of U251 cells and in the endoplasmic reticulum and plasma membranes of LNCaP cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Calnexin

Target: Calnexin (CANX)

Andere Bezeichnung: CANX

Hintergrund: CANX, calnexin, CNX, FLJ26570, IP90, P90, major histocompatibility complex class I antigen-binding protein p88

Gen-ID: 821, 12330, 29144

NCBI Accession: NP_001737

Pathways: MAPK Signalweg, Thyroid Hormone Synthesis