PDK1 Antikörper (AA 107-336)

Produktnummer ABIN5708184

Der Kaninchen Polyklonal Anti-PDK1-Antikörper wurde für WB, ELISA und IHC (p) validiert. Er ist geeignet, PDK1 in Proben von Human und Ratte zu detektieren.

Kurzübersicht für PDK1 Antikörper (AA 107-336) (ABIN5708184)

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PDK1 Antikörper

Bindungsspezifität: AA 107-336

Aufreinigung: Antigen affinity purified

Immunogen: A recombinant human protein corresponding to amino acids V107-Y336 was used as the immunogen for the PDK1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PDK1 antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL, IHC (FFPE): 1-2 μg/mL, Direct ELISA: 0.1-0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the PDK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu PDK1

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Andere Bezeichnung: PDK1 / Pyruvate Dehydrogenase Kinase 1

Hintergrund: PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93 % identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.

UniProt: Q15118

Pathways: PI3K-Akt Signalweg, RTK Signalweg, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Warburg Effekt