Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch GFAP in IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus und Schwein.
The optimal dilution of the recombinant GFAP antibody for each application should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
1 mg/mL in 1X PBS, BSA free, sodium azide free
Konservierungsmittel
Azide free
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store the recombinant GFAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Target
GFAP
(Glial Fibrillary Acidic Protein (GFAP))
Andere Bezeichnung
GFAP / Glial Fibrillary Acidic Protein
Hintergrund
This mAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.