GFAP Antikörper

Produktnummer ABIN6939502

Dieser Maus Monoklonal Antikörper detektiert spezifisch GFAP in WB, IHC, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus, Rind (Kuh), Schwein, Huhn, Kaninchen, Meerschweinchen und Hamster.

Kurzübersicht für GFAP Antikörper (ABIN6939502)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Schwein, Huhn, Kaninchen, Meerschweinchen, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: GA-5

Produktdetails anti-GFAP Antikörper

Verwendungszweck: GFAP (Astrocyte & Neural Stem Cell Marker) Antibody

Spezifität: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: GFAP isolated from pig spinal cord

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP

Hintergrund:

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

T98G cells (FACS). Brain or Astrocytoma (IHC). Human Brain, Mouse Brain, Rat Brain, Guinea Pig Brain or Hamster Brain tissue lysates (WB).

Molekulargewicht: ~50kDa

Gen-ID: 2670, 514227

UniProt: P14136