IL1A Antikörper

Produktnummer ABIN5706830

Hersteller Produkt- Nr.: 209-401-302-0100

Produktdetails anti-IL1A Antikörper

Target: IL1A (Interleukin 1 alpha (IL1A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Hersteller Produkt- Nr.: 209-401-302-0100

Hersteller: Rockland

Verwendungszweck: IL-1 Alpha Antibody

Kreuzreaktivität (Details): This antibody is primarily directed against the 17,000 MW human IL-1a and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1a in the same formats using similar dilutions.

Produktmerkmale: Synonyms: Interleukin 1 alpha precursor antibody, rabbit anti-Interleukin1 alpha antibody, rabbit anti-IL-1 Alpha Antibody, Pre-interleukin 1 alpha antibody

Sterilität: Sterile filtered

Immunogen:

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli.  The MW of the recombinant IL-1a was 17,000.  This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 1:100 to 1:200

Application Note: This IgG fraction antibody of anti-Human IL-1a has been tested for use in immunoblotting. This antibody is suitable for use in ELISA, neutralizations, immunohistochemistry, and flow cytometry. It recognizes the 17,000 MW mature IL-1a.  Reactivity in other immunoassays is unknown.

Neutralization Dilution: User Optimized

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: IL1A (IL1A Produkte)

Hintergrund: Background: Interleukin 1 alpha is produced by activated macrophages. Anti-IL-1 alpha antibody is ideal for investigators involved in Immunology research.

Gen-ID: 3552

UniProt: P01583

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints