Leptin Antikörper

Produktnummer ABIN5706824

Produktdetails anti-Leptin Antikörper

Target: Leptin (LEP)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Leptin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktmerkmale:

Synonyms: rabbit anti-leptin antibody, LEP antibody, Leptin Murine Obesity Homolog antibody, Leptin Precursor Obesity Factor antibody, OB antibody, Obese Protein antibody

Background: Anti-Leptin antibody detects leptin protein. Leptin may function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Obesity associated leptin is the product of the OB gene and has been identified with Type II diabetes. Anti-leptin Antibody is ideal for investigators involved in Cell Signaling, Obesity research, aging research and Signal Transduction research.

Gene Name: Leptin

Aufreinigung: Anti-Leptin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  The antibody will recognize leptin from mouse and human.  Reactivity with leptin from other sources is unknown.

Immunogen:

Immunogen: Anti-Leptin antibody was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: This IgG fraction of anti-Mouse Leptin was tested for use in ELISA and immunoblotting.  Reactivity in other immunoassays is unknown. The antibody will recognize recombinant and native 16 kDa leptin from mouse and human.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 12 months

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: Leptin (LEP Produkte)

Synonyme: ob antikoerper, obese antikoerper, LEPD antikoerper, OB antikoerper, OBS antikoerper, leptin antikoerper, Lep antikoerper, LEP antikoerper, lep antikoerper

Gen-ID: 16846

UniProt: P41160

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process